Kemtrak UV Analyzers

Kemtrak Photometer LCD displayThe DCP007-UV analyzer is an advanced dual wavelength photometer designed to accurately measure absorbance in the UV region of the electromagnetic spectrum. Optical fibers are utilized to transmit light from a source within the DCP007-UV instrument through an in-line sample cell and back again to a sensitive detection system to calculate the concentration of many substances in real time. The absence of electrical power in the light transmission system allows sample cells to be fitted in hazardous areas without any concern for electrical certification and approval. The DCP007-UV is an excellent and cost effective solution for measuring flow stream absorbance and concentration in a process environment in place of far more expensive scanning type analyzers.

 

Traditional UV photometers that use mercury vapor lamps that continuously expose the process stream to high intensity broad spectrum UV radiation and heat generated by the lamp. This can result in destruction of valuable in-stream product and produce unknown and potentially hazardous breakdown products. Traditional UV photometers also suffer from systematic drift requiring constant adjustment due to optical filter erosion caused by high intensity UV radiation from the lamp and by continual lamp output change with age.

 

The Kemtrak DCP007-UV process analyzer uses state-of-the-art LED light sources at the exact wavelength required for the analysis. Light reaches the process through precision fiber optics and is delivered with energy levels thousands of times less than that of a traditional UV photometer. The ultra-low modulated power UV LED light source in the Kemtrak DCP007-UV process analyzer does not systematically drift or expose the product stream to high levels of UV radiation and temperature. LED light sources are available at discrete wavelengths from 250nm to 400nm in the UV region, ensuring any specific wavelength requirement can be serviced.

 

A proprietary dual wavelength, four-channel measurement technique and advanced digital electronic design allows for deep UV absorbance measurement up to 5AU (in a 1cm optical path length) at up to two discrete wavelengths. A selection of shorter optical path-lengths allow for even deeper absorbance measurements.

 

Convenient zero dead-volume hygienic measurement cells contain no electronics or moving parts and are well suited for hazardous environments. In-line verification and QA is made possible using an insertable cuvette style certified filters or liquids. This simple method utilizes the same traceable or prepared liquid standards used for laboratory benchtop instrumentation verification and calibration and provides a direct correlation to offline measurement techniques for added confidence in measurement.

 

Typical applications for the DCP007-UV are:

  • Chromatographic Separation
  • Micro- and nano- filtration systems
  • Concentration of aromatic solvents

 

The DPC007-UV in-line process analyzer will give a measurement performance you would never have thought possible in an on-line UV analyzer at a price point similar to other inferior systems.

Is Kemtrak Right for Your Application?

Let Kemtrak give you a solution – download an application data sheet, fill in your process specifications and send it to us. We’ll come back to you with a configuration that will suit your needs.

Traceable Validation Accessory

The optional Kemtrak NIST-traceable validation filter holder is supplied either fitted to the optical flow cell or as a standalone accessory in the optical fiber chain. It permits the use of either NIST traceable reference standards or liquid samples to quickly check instrument performance on a regular basis to ensure the photometer is operating within satisfactory parameters without having to remove the flow cell from the process line.

The Kemtrak NIST-traceable validation filter holder is designed to use industry standard 10mm cuvette style NIST-traceable absorbance filters, the same type as used on many standard laboratory spectrophotometers.

Benefits of using a Kemtrak NIST-traceable validation filter holder include:

  • Fast verification of measurement accuracy
  • Industry standard 10mm cuvette size
  • Same set of standard NIST filters as used in the laboratory
  • Cross validation between laboratory spectrophotometer and Kemtrak in-line industrial process photometers
  • Liquid samples can be measured
  • Recognized by international quality systems such as GLP, ISO 9000 and ISO/IEC 17025

Biotech Application Wavelengths

This table give a short summary of some of the wavelengths typically used in biotechnology applications such as chromatography and filtration

WavelengthAbsorbing SpeciesApplicationsNotes
254nmNucleotidesNucleotide bases, DNA, RNAThis is the best wavelength for nucleic acids.
280nmAromatic amino acidsProteinsThis is the traditional and therefore the most frequently used wavelength by biochemists as it is best for seeing aromatic amino acids (i.e.: tryptophan, tyrosine, phenylalanine). Proteins with few of these amino acids in them may not show well.
313nmConjugated Ring SystemsCertain Vitamins, antibiotics
365nmConjugated Ring SystemsSome steroids, NADH, NADPH Flavoproteins, Bacteriochlorophylls
405nmHeme groups, porphyrin cyto-ring systemsFerroproteins (hemoglobins), chrom porphyrin derivatives

Technology Overview

The Kemtrak DCP007 photometer measures at two wavelengths. A primary “absorbing” wavelength is used to measure the concentration of the chemical being monitored while a “non absorbing” reference wavelength is used to compensate for turbidity, air bubbles and/or fouling.

The Kemtrak DCP007 photometer uses precision fiber optics to shine a precisely focused monochromatic light through the process medium and back to a photo-detector.

The attenuation of the transmitted light beam caused by NIR absorbing substances in the process medium is mathematically described by the Beer-Lambert law.

Applying the Beer-Lambert law (below), the concentration of the sample can be calculated.

A = ε l c

where:

A = absorbance (= – log transmittance)

ε = molar absorptivity coefficient

L = optical path length

C = concentration of absorbing species