Many optical biomass sensors rely on light transmission measurement, where light must pass through the sample to a detector positioned opposite the source. In high-density fermentation, the medium becomes so opaque that light cannot penetrate it, leading to a measurement "plateau" where the sensor cannot trend increasing biomass density.
The Kemtrak TC007-NIR backscatter photometer circumvents this by positioning the light source and the detector on the same plane. It measures the intensity of Near-Infrared (NIR) light scattered at a 180° angle back through the same window. As the concentration of cells increases, the amount of reflected light increases proportionally, providing a linear and reliable signal even in extremely high biomass density environments that blind transmission sensors.
Beyond its measurement range, the TC007 is built for the rigors of a modern bioprocessing facility. The instrument employs high-precision fiber optics to connect the control unit to the measurement point, meaning the probe itself contains no sensitive electronics. This design allows the probe to withstand extreme temperatures (up to 250°C) and the aggressive chemicals used in CIP (Clean-in-Place) and SIP (Steam-in-Place) cycles without drift or degradation. The use of scratch-resistant sapphire windows further ensures long-term reliability and minimal maintenance.
To provide the cleanest possible data for process control, the TC007 includes intelligent signal processing that automatically compensates for window fouling and sample color changes. It also features integrated bubble and particle noise filtering, preventing the common "spikes" in data caused by aeration in the fermenter. With support for both discrete outputs and digital communication protocols, the TC007 integrates seamlessly into automated control systems to optimize yield and batch consistency.
TC007 Analyzers
The Kemtrak TC007 turbidimeter platform provides the ultimate in accurate and drift free online biomass measurement. TC007 instruments require minimal maintenance and integrate into the lab environment as well as pilot/production control systems.
Kemtrak TC007 benefits include:
- Real-time in-line biomass measurement
- Suitability for sterile/GMP facilities
- Near-zero maintenance
- Reliable, robust infrared LED light source providing drift-free operation
- Analog output (0/4-20 mA)
- Modbus (slave) over TCP/IP
- Local and web-based graphical user interface (TCP/IP)
TC007-SCAT
The TC007-SCAT unit is typically used in mammalian cell culture and perfusion batch applications with total suspended solids up to ~5%. Measurement is made using a single window backscatter probe. Light shines into the process and the close proximity of the cell matter passing over the window surface causes scattering, returning a signal back through the window and to the analyzer.
| Operating Wavelength | 850 nm |
| Measurement Range | 0…50 g/L (0…5% total suspended solids) |
| Resolution | 0.001% total suspended solids |
| Accuracy | +/- 2% of reading |
TC007-NIR-SCAT
The TC007-NIR-SCAT unit is ideal for bacterial and other very high cell density applications with high total suspended solids. The NIR-SCAT version has a longer wavelength of operation which allows a much higher level of solids measurement, even in dark backgrounds.
| Operating Wavelength | 1440 nm |
| Measurement Range | 0…>100+ g/L (0…>>20% total suspended solids) |
| Resolution | 0.001% total suspended solids |
| Accuracy | +/- 2% of reading |
Inline Probes
TC007 probes are maintenance-free and have scratch-resistant sapphire windows. Probes are connected directly to the TC007 analyzer with fiber optic cables and without onboard electrical or electronic components, are suitable for hazardous area installation.
All Kemtrak backscatter probes include:
- Suitability for sterile/GMP facilities
- Near-zero maintenance
- High quality finish
- FDA/USP VI approved materials
- Autoclavable and SIP/CIP compatible measurement probes
Backscatter Immersion Probe
The backscatter immersion probe is designed with the same footprint as standard pH probes and fits into standard bioreactor top plate entries. A variety of lengths is available to suit the height of different volume vessels. It connects directly to TC007-NIR/NIR-SCAT electronics.
| Measurement Range | 0 - >90% total suspended solids |
| Available Materials | 316L Stainless Steel, Hastelloy C-22 |
| Surface Finish | Ra <0.38 micron, 15microinch |
| Available lengths | 120, 225, 325, 425 mm |
| Vessel Connection | Pg13.5 |
| Available FDA/USP VI Elastomers | FKM, FFKM, EPDM |
| Operating Temp. | Max 200°C / 392°F |
| Operating Pressure | Max 50 bar / 725 psi |
| Fiber Optic Cable Length | 5m / 16.4ft (standard). Up to 20m/66ft available |
Backscatter Sanitary Clamp Probe
The backscatter sanitary clamp probe is designed to fit into sanitary clamp ports in vessel side walls and flowing pipes. It connects directly to TC007-NIR/NIR-SCAT electronics.
| Measurement Range | 0 - >90% total suspended solids |
| Available Materials | 316L Stainless Steel |
| Surface Finish | Ra <0.38 micron, 15microinch |
| Available lengths | Compatible with ISO 2852 50.5mm/2” OD flange fittings (DN25, DN32, DN40, 1" and 1½") |
| Available FDA/USP VI Elastomers | FKM, FFKM, EPDM |
| Operating Temp. | Max 200°C / 392°F |
| Operating Pressure | Max 25 bar / 363 psi |
| Fiber Optic Cable Length | 5m / 16.4ft (standard). Up to 20m/66ft available |
Calibration and Verification Tools
Calibration and verification tools for Kemtrak TC007 Analyzers are available to make calibration and verification quick, easy and repeatable. Kemtrak calibration standards are available for all modes of TC007 operation with a range of different values to ensure that they are working to specification.
When applied, Kemtrak Cal tool kits simulate a known traceable measurement response. Kits are supplied in a protective case with supporting documentation.
CalCaps Verification Tool
Kemtrak CalCaps a provide a foolproof method of quickly and easily validating the calibration of TC007-SCAT/NIR-SCAT biomass analyzers using 12mm diameter pH probe style or Triclamp® flanged insertion back scatter probes. CalCaps are liquid free and simulate known conditions when applied to the probe tip.
| Response Values | Dark (No backscatter) 4000 FBU (formazin backscatter units) |
| Use With: | Kemtrak backscatter probe connected to a TC007 operating in SCAT/NIR-SCAT mode |
| Carrying Case | Included |
| Certification | Included |
TC007 Frequently Asked Questions
The TC007 uses backscatter measurement, which is well suited for high cell density applications where traditional absorbance methods lose sensitivity. It provides stable, real-time readings across a very wide concentration range, including very dense fermentations.
Have more questions? Take a quick moment to get in touch with our expert sales engineers and technologists and we'll guide you to a solution.
No matter the application or industry, South Fork Instruments can develop a superior optical sensor-based measurement solution.
We have provided Biomass Monitor solutions to clients in such industries as food and beverage, chemical processing, pharmaceutical manufacturing, and biotechnology, and can assist you in developing and effective and efficient means of fulfilling your measurement needs.
SOUTH FORK INSTRUMENTS
3845 Buffalo Road
Auburn, CA 95602
Tel: (+1) 925-461-5059
